№ files_lp_3_process_9_44641
File format: docx Character count: 9988 File size: 1030 KB
A detailed protocol for culturing human induced pluripotent stem cells (hiPSCs), differentiating them into neurons and microglia, and troubleshooting common issues.
Note: Year
Theme: Cell culture, differentiation
Document Type: Protocol
Target Audience: Researchers in cell biology, stem cell culture
Price: 8 / 10 USD
The file will be delivered to the email address provided at checkout within 12 hours.

Don’t have cryptocurrency yet?

You can still complete your purchase in a few minutes:
  1. Buy Crypto in a trusted app (Coinbase, Kraken, Cash App or any similar service).
  2. In the app, tap Send.
  3. Select network, paste our wallet address.
  4. Send the exact amount shown above.
After sending, paste your TXID (transaction ID) and your email to receive the download link. Need help? Contact support and we’ll guide you step by step.

The final amount may vary slightly depending on the payment method.
The file will be sent to the email address provided at checkout within 24 hours.
The product description is provided for reference. Actual content and formatting may differ slightly.
Supplementary Table S1. Representative Traditional Chinese Medicinal Herbs Containing Natural Compounds for Regulating Microglial Response
Document type: Supplementary scientific table
Subject: Traditional Chinese medicinal herbs and natural compounds regulating microglial response
Field of study: Pharmacology and neuroinflammation research
Content elements: Botanical name, Chinese name, traditional functions, clinical applications, active compounds, references
Medical focus: Microglial modulation and related diseases
Diseases mentioned: Ischemic stroke, cancer, diabetes, cardiovascular diseases, neurodegenerative diseases, inflammatory disorders
Compounds highlighted: Ligustilide, Artesunate, Arctigenin, Astragaloside IV, Curcumin, Berberine, Ginsenosides, Resveratrol, Tanshinone IIA, Triptolide
Source type: Academic publication supplementary material
Language: English
Methods and Experimental Model Details for SMCxPRO pUb Assay and α-Synuclein PFF Studies in Mice, Rats, and Primary Neuronal Cultures
Document type: Materials and Methods section
Species: C57Bl6/J mice; Sprague Dawley rats
Animal source: The Jackson Laboratories (Bar Harbor, Maine); Zhejiang Vital River Laboratory Animal Technology Co., Ltd.
Experimental facilities: BioDuro-Sundia (Shanghai, China)
Ethics approval: IACUC approved protocols
Regulatory compliance: Animal Welfare Act (9 CFR Parts 1–3); Guide for the Care and Use of Laboratory Animals (8th Edition); AAALAC accreditation (001516)
Cell lines: YPMK; YPMK PINK1 KO; EPF1; HEK293 PINK1 KO; SK-OV-3; ΔOTC
Primary cultures: Mouse hippocampal and cortical neurons (E16, A53T α-synuclein M83 mice)
Key assay: SMCxPRO pUb assay
Key reagents: Rabbit anti-pS65-Ub antibody (CST E2J6T); Mouse anti-Ub antibody (CST P4D1); K48 pUb tetramers (RnD Systems UC-250)
Instrumentation: SMCxPRO system; Agilent Seahorse XFe96 analyzer; ImageXpress Micro Confocal High-Content Imaging System
Experimental focus: α-synuclein preformed fibrils (PFFs); mitochondrial respiration; mitophagy measurement (MT-mKeima)
Data availability: Available from corresponding author upon reasonable request under Material Transfer Agreement
Code availability: No original code reported
Synaptic Responses, Neuronal Circuitry and Neuromodulation Using the Crayfish: Student Laboratory Exercises
Year: 2008
Region / City: Not specified
Subject: Neuroscience, Physiology
Document Type: Student Laboratory Exercise
Institution: Not specified
Author: Not specified
Target Audience: Students, Researchers in Neuroscience
Period of Activity: Not specified
Date of Approval: Not specified
Date of Changes: Not specified
Contextual Description: Laboratory exercises for understanding synaptic transmission and neuromodulation through crayfish models, aimed at students studying neuronal physiology.
Topics: hiPSC Microglial Neuronal