№ files_lp_4_process_3_115580
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Detailed protocols for cell culture, genetic manipulation, and molecular analyses in glioblastoma stem cells, including genome-wide expression and ChIP-Seq experiments.
Year: 2017
Institution: The University of Texas MD Anderson Cancer Center
Region / City: Houston, TX, USA
Document type: Supplementary materials
Subject: Glioblastoma stem cells, REST and DRD2 pathways
Methods: Cell culture, immunofluorescence, RNA preparation, qRT-PCR, chromatin immunoprecipitation, genome-wide expression analysis, Western blot, ChIP-Seq
Target audience: Researchers in molecular biology and oncology
Approval: Institutional review board approved
Experimental models: HR-GSC1, HR-GSC2, LR-GSC cell lines
Key reagents: Lentiviral vectors, antibodies, Trizol, microarrays
Analysis software: Agilent GeneSpring GX, Ingenuity Pathway Analysis
Supplementary content: Materials and methods, figure legends
Price: 8 / 10 USD
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