№ files_lp_3_process_7_004092
File format: docx
Character count: 16652
File size: 45 KB
This SOP provides detailed instructions for preparing whole cell protein extracts to analyze differential protein expression in proteomics studies, focusing on lysing cells, protein extraction, and preparing samples for mass spectrometry analysis.
Year:
2023
Region / City:
United States
Subject:
Proteomics, Sample Preparation
Document Type:
Standard Operating Procedure (SOP)
Institution:
Proteomics and Metabolomics Core
Author:
David Kakhniashvili, Ph.D.
Target Audience:
Researchers in proteomics, molecular biology, bioinformatics
Effective Period:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Price: 8 / 10 USD
The file will be delivered to the email address provided at checkout within 12 hours.
The file will be delivered to the email address provided at checkout within 12 hours.
Don’t have cryptocurrency yet?
You can still complete your purchase in a few minutes:- Buy Crypto in a trusted app (Coinbase, Kraken, Cash App or any similar service).
- In the app, tap Send.
- Select network, paste our wallet address.
- Send the exact amount shown above.
The final amount may vary slightly depending on the payment method.
The file will be sent to the email address provided at checkout within 24 hours.
The product description is provided for reference. Actual content and formatting may differ slightly.
Year:
2014-2018
Research focus:
Breast cancer molecular profiling and survival analysis
Document type:
Supplementary methods
Institution:
National Cancer Institute, Center for Cancer Research; Affymetrix; Illumina; Qiagen
Techniques:
Gene expression profiling, RNA sequencing, mass spectrometry-based proteomics, LASSO Cox regression
Sample type:
Human breast tumor tissues and treated cell lines
Number of samples:
61 tumors in discovery set; additional cell line experiments
Data analysis software:
R packages (Oligo, limma, DESeq2, survival, glmnet, glmpath, survcomp), Picard, Trimmomatic, TopHat, HTSeq, Proteome Discoverer, SEQUEST HT
Genes analyzed:
28,869 genes
Proteins identified:
~4,000 with ≥2 peptides and ≥15% tissue representation
Metabolites analyzed:
398 metabolites
Treatment conditions:
Deoxycholate 20 µM and 50 µM in cell lines
Statistical methods:
Wald statistics, Benjamini-Hochberg adjustment, LASSO feature selection, Kaplan-Meier survival analysis, concordance index (C-index)
Clinical variables:
Age, race/ethnicity, disease stage and grade, node status, menopausal status, neoadjuvant/hormone/chemotherapy
Year:
2022
Region / City:
Duluth, MN
Subject:
Genomics, Proteomics, Actinobacteriophages
Document Type:
Abstract
Institution:
College of St. Scholastica
Author:
Ella K Achterkirch, Julia L Carlson, Jonah C Giermann, Ellie J Peddle, Harrison S Reinke, Rachel A Rhein, Houa Thao, Daniel E Westholm
Target Audience:
Researchers in genomics and proteomics
Period of Validity:
N/A
Approval Date:
N/A
Date of Modifications:
N/A
Context:
This is an academic abstract detailing the comparative genomics and proteomics of two actinobacteriophages, LittleMunchkin and Venti, focusing on their genome sequences, potential genes, and lysogeny analysis.
Facility Director:
Anna Schmoker, Ph.D.
Facility Location:
Rubin 7, Dartmouth Hitchcock Medical Center
Contact email:
[email protected]
Contact phone:
(603) 646-5573
Year:
2024
Region / City:
Dartmouth, NH
Field:
Proteomics, Mass Spectrometry
Document Type:
Facility Description
Institution:
Dartmouth College
Target Audience:
Researchers at Dartmouth College
Action Period:
From November 2024
Approval Date:
November 2024
Changes Date:
Not specified
Year:
2026
Region / City:
New York
Topic:
Mass spectrometry, proteomics
Document type:
Research Resource
Institution:
Albert Einstein College of Medicine
Author:
Not specified
Target audience:
Researchers, scientists in proteomics
Period of validity:
Ongoing
Approval date:
Not specified
Date of changes:
Not specified
Year:
2014-2018
Research focus:
Breast cancer molecular profiling and survival analysis
Document type:
Supplementary methods
Institution:
National Cancer Institute, Center for Cancer Research; Affymetrix; Illumina; Qiagen
Techniques:
Gene expression profiling, RNA sequencing, mass spectrometry-based proteomics, LASSO Cox regression
Sample type:
Human breast tumor tissues and treated cell lines
Number of samples:
61 tumors in discovery set; additional cell line experiments
Data analysis software:
R packages (Oligo, limma, DESeq2, survival, glmnet, glmpath, survcomp), Picard, Trimmomatic, TopHat, HTSeq, Proteome Discoverer, SEQUEST HT
Genes analyzed:
28,869 genes
Proteins identified:
~4,000 with ≥2 peptides and ≥15% tissue representation
Metabolites analyzed:
398 metabolites
Treatment conditions:
Deoxycholate 20 µM and 50 µM in cell lines
Statistical methods:
Wald statistics, Benjamini-Hochberg adjustment, LASSO feature selection, Kaplan-Meier survival analysis, concordance index (C-index)
Clinical variables:
Age, race/ethnicity, disease stage and grade, node status, menopausal status, neoadjuvant/hormone/chemotherapy
Year:
2025
Region / city:
N/A
Topic:
Pension and fiduciary services, End Product (EP) codes
Document type:
Training guide
Organization / institution:
N/A
Author:
N/A
Target audience:
Entry-level PMC VSRs
Period of validity:
N/A
Approval date:
N/A
Date of changes:
N/A
Year:
2026
Note:
Region / City
Topic:
Pension and Fiduciary Services
Document Type:
Training Material
Target Audience:
Entry-level PMC VSRs
Lesson Duration:
6 hours
Prerequisite Training:
Phases 1–5.6
Lesson Objectives:
Recognize and process initial vs supplemental claims, develop claims, confirm claim characteristics, determine evidence sufficiency, prepare decisions.
Relevant Legislation:
Appeals Modernization Act (AMA), signed on August 23, 2017, effective February 19, 2019
References:
M21-1 II.iii.1.A.1.a, M21-1 X.ii.2.A.3, M21-4 Appendix B
Key Concepts:
Initial Claims, Supplemental Claims, Effective Date Protection, New and Relevant Evidence
Year:
2026
Region / City:
Hot Springs National Park
Theme:
Adaptive Reuse, Historic Preservation
Document Type:
Request for Expressions of Interest (RFEI)
Organization / Institution:
National Park Service (NPS)
Author:
National Park Service
Target Audience:
Individuals, businesses, educational institutions, government agencies, non-profit and for-profit organizations
Period of Validity:
Indefinite
Approval Date:
N/A
Amendment Date:
N/A
Year:
2022
Region / City:
York, PA
Document Type:
Official Correspondence
Organization / Institution:
Department of Veterans Affairs
Author:
VA Regional Office
Target Audience:
David Smith (Veteran)
Period of Effectiveness:
March 1, 2021
Approval Date:
April 23, 2022
Amendment Date:
None
Evidence Reviewed:
Statement in support of claim, VA Form 21P-8416 Medical Expense Report, Care Expense Statement
Requested Information:
VA Form 21P-0969, Bill of sale for primary residence
Deadline for Submission:
60 days from April 23, 2022
Additional Instructions:
Contact details for further inquiries
Context:
Document is an official letter notifying a veteran of proposed adjustments to pension benefits due to an increase in net worth, detailing the rights to submit evidence or request a hearing.
Year:
2025
Region / City:
Not specified
Topic:
Pension and Fiduciary Services, Training
Document Type:
Trainee Guide
Organization:
Not specified
Author:
Not specified
Target Audience:
Entry-level PMC VSRs
Duration:
6 hours
Date of Approval:
Not specified
Date of Changes:
Not specified
Year:
2025
Jurisdiction:
England and Wales
Document Type:
Court Judgment
Court:
Court of Appeal
Case Reference:
EWCA Civ 1126
Subject:
Anonymity Orders, Personal Injury Claims
Involved Organization:
Personal Injuries Bar Association (PIBA)
Key Legal Precedent:
JX MX v Dartford and Gravesham NHS Trust [2015] EWCA Civ 96
Relevant Procedure Rules:
CPR 21.10, PF10
Authors / Contributors:
Sarah Crowther KC, Anya Proops KC, Stephen Cottrell, Hannah Ready, Greg Cox, Edward Stansfield
Audience:
Legal practitioners in personal injury law