№ lp_1_2_19802
File format: docx Character count: 14737 File size: 214 KB
Technical protocol for labeling proteins with europium using DELFIA® Eu-N1 ITC chelate for subsequent measurement in time-resolved fluoroimmunoassays.
Catalog#: 1244-302
Intended use: Research use only
Kit contents: Labelling reagent (Eu-N1 ITC chelate), Eu-Standard, Enhancement Solution, Stabilizer (BSA), uncoated microtitration plate, DELFIA Assay Buffer, Wash Concentrate
Required but not supplied: Labelling buffer, chromatographic system, elution buffer, column decontamination buffer, precision pipettes, spectrophotometer, automatic shaker, VICTOR™ Multilabel Counter
Warnings: Contains sodium azide, handle Eu3+ solutions carefully, dispose of waste according to local regulations
Labeling conditions: Protein in amine-free buffer, 50–100 mM sodium bicarbonate/carbonate buffer, pH 9–9.3, +4°C, overnight reaction
Expected labeling yield: 1–12 Eu3+ per IgG depending on molecular weight and type (monoclonal/polyclonal)
Storage: Thermodynamically stable for long-term storage of labeled proteins
Author/Manufacturer: PerkinElmer
Price: 8 / 10 USD
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