№ files_lp_4_process_2_48449
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Detailed experimental protocols for generating, differentiating, and analyzing human mast cells and evaluating the biological activity of the humanized monoclonal antibody KTN0158.
Year: 2026
Location: United States
Subject: Immunology, Monoclonal Antibodies, Mast Cell Biology
Document Type: Research Supplement / Methods Section
Institution: Kolltan Pharmaceuticals
Authors: London et al.
Target Audience: Scientific researchers in immunology and pharmacology
Experimental Period: 2026
Cell Types: Human CD34+ progenitor cells, PBMCs, mast cells
Key Techniques: Flow cytometry, antibody assays, cell differentiation, KIT phosphorylation
Reagents: KTN0158, KTN0062C, KTN0209, KTN0235, Imatinib, Nilotinib, IL-3, IL-6, SCF, LDL
Price: 8 / 10 USD
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Generation and characterization of humanized CCR8 knock-in mice and transfected cell lines for in vitro binding assays
Year: 2026
Region / City: Japan
Subject: Immunology, Gene editing, Chemokine receptors
Document type: Supplementary material / Methods
Institution: FASMAC, Takara Bio, Integrated DNA Technologies, Thermo Fisher Scientific
Authors: Not specified
Target audience: Researchers in molecular biology and immunology
Experimental models: HEK293T, Rat-1, KHYG-1, Ramos, CHO/G16 cells; hCCR8 knock-in mice
Techniques: Gene transfection, CRISPR/Cas9 knock-in, flow cytometry, in vitro binding assays
Reagents: Antibodies for human and mouse chemokine receptors, Puromycin, G418
Period of study: Not specified
Date of preparation: 2026
Topics: KTN0158 anti-KIT humanized