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Supplementary methodological section of a biomedical research study detailing reagents, experimental protocols, cell culture systems, and analytical techniques used to investigate ICOSLG signaling and regulatory T-cell responses in glioblastoma.
Document Type: Supplementary research methods
Research Field: Tumor immunology
Disease Focus: Glioblastoma multiforme
Molecular Focus: ICOSLG-mediated signaling and IL-10–producing regulatory T cells
Experimental Models: Human glioma stem cells, murine RasR2 glioma-initiating cells, CD4+ naïve T cells
Biological Materials: Patient-derived neurospheres, peripheral blood mononuclear cells
Techniques: Flow cytometry, immunofluorescence staining, luciferase reporter assay, siRNA transfection, hypoxia induction, cytometry by time-of-flight (CyTOF)
Cell Culture Conditions: Defined DMEM/F12 medium with B27, heparin, EGF, and bFGF
Key Molecular Targets: ICOSLG, NF-κB, Foxp3, STAT5, PD-L1, HLA molecules
Reagents and Antibodies: Commercial reagents and antibodies from multiple biotechnology suppliers
Associated Experiments: Analysis of ICOSLG expression, NF-κB promoter activity, tumor-conditioned medium effects on T cells
Source Material: Surgical glioma specimens and established experimental cell lines
Price: 8 / 10 USD
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Glioblastoma Multiforme: References
Note: Year
Author: Willie Ching, PT, DPT, Board Certified Specialist in Neurologic Physical Therapy
Contextual Description: A fact sheet referencing various studies and research articles on Glioblastoma Multiforme, including treatment options and clinical trials.
Glioblastoma Multiforme: Background and Epidemiology/Statistics
Author: Willie Ching, PT, DPT, Board Certified Specialist in Neurologic Physical Therapy
Organization: American Physical Therapy Association (APTA) – Neurology Section
Document Type: Fact Sheet
Medical Condition: Glioblastoma Multiforme (GBM)
Target Audience: Healthcare professionals, patients, caregivers
Diagnostic Methods: MRI, biopsy
Treatment Methods: Surgery, radiation therapy, chemotherapy (Temozolomide), tumor-treating fields (TTFields)
Epidemiology: Incidence 2–3 per 100,000 adults per year, median age 64, higher prevalence in men and Caucasians
Survival Rates: 26–33% at 2 years, 4–5% at 5 years
Risk Factors: Ionizing radiation, certain chemical exposures, genetic syndromes (neurofibromatosis, tuberous sclerosis, Li-Fraumeni, retinoblastoma, Turcot syndrome)
Contact: ANPT, Phone 952.646.2038, [email protected]
Note: , www.neuropt.org
Glioblastoma Multiforme: Role of Physical Therapy and Research Updates
Year: 2023
Region / City: United States
Topic: Glioblastoma Multiforme, Physical Therapy, Research Updates
Document Type: Fact Sheet
Organization: ANPT
Author: Willie Ching, PT, DPT, Board Certified Specialist in Neurologic Physical Therapy
Target Audience: Healthcare professionals, patients with GBM, caregivers
Period of Action: Ongoing
Approval Date: Not specified
Date of Changes: Not specified
Topics: ICOSLG-mediated IL-10–Producing Multiforme