№ files_lp_4_process_3_133354
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Supplementary tables listing primer sequences used for dsRNA synthesis, quantitative PCR, and RNA-seq validation of differential gene expression in Oithona similis.
Year: 2026
Organism: Oithona similis
Type of document: Supplementary material
Methods: PCR, qRT-PCR, RNA-seq
Content: Primer sequences for dsRNA synthesis, qRT-PCR assays, and DEG validation
Genes analyzed: Multiple O. similis gene clusters
Target molecules: dsAllim, dsOslim, dsGFP
Purpose: Gene expression analysis and validation
Laboratory techniques: Molecular biology, genetic analysis
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List of primer sequences used for qRT-PCR analysis.
Year: 2023
Region / city: Not specified
Subject: Gene expression analysis, pre-eclampsia, fetal growth restriction
Document type: Research data
Organization / institution: Not specified
Author: Not specified
Target audience: Researchers in molecular biology and genomics
Period of validity: Not specified
Approval date: Not specified
Date of changes: Not specified
Experimental Design and qRT-PCR Analysis of TBHQ and Alcohol Effects on CTX TNA2 Astrocytes
Year: Not specified
Cell line: CTX TNA2 astrocytes
Experimental groups: Control; TBHQ (40 µM TBHQ + alcohol); Alcohol (75 mM)
Treatment duration: 24 h TBHQ pretreatment followed by 24 h alcohol exposure
Biological replicates: 3 per group
Technical replicates: 2 wells per sample
Sample type: Total RNA from cultured astrocytes
Culture conditions: 6-well plates; 2×105 cells/ml
RNA extraction method: TRIzol® reagent
RNA storage: Dissolved in DEPC-treated water; stored at -80℃
RNA quality assessment: OD260/280 between 1.8 and 2.0; 28S/18S ratio 1.7–2.1
Reverse transcription kit: PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time RR047A), Takara
qPCR reagent: TB Green Premix Ex Taq II (Tli RNaseH Plus), Code No. RR820A
Target gene: NM_001009920 glutathione S-transferase Yc2 subunit Gsta5 (Yc2)
Reference gene: GAPDH
qPCR instrument: Roche LightCycler96
Statistical analysis: One-way ANOVA with post hoc least significant difference test
Significance threshold: P < 0.05
Software: IBM SPSS Statistics 21
Supplementary Table S1: primers sequences for qRT-PCR
Year: 2026
Region / city: Not specified
Theme: Genetics, Molecular Biology
Document Type: Research Supplementary Table
Organization / Institution: Not specified
Author: Not specified
Target Audience: Researchers in Molecular Biology
Period of Action: Not applicable
Approval Date: Not specified
Date of Changes: Not specified
Context: A supplementary table listing primers and sequences used for qRT-PCR, along with associated experimental data on gene knockdown and antibody information.
Supplementary Tables: Details regarding vector construction and qRT-PCR primers
Gene: Gene
Year: Not specified
Region / City: Not specified
Topic: Primer sequences for vector construction and qRT-PCR
Document Type: Scientific supplementary data
Institution: Not specified
Author: Not specified
Target Audience: Researchers in molecular biology
Period of Effect: Not specified
Approval Date: Not specified
Amendment Date: Not specified
Context: Document provides detailed sequences for vector construction primers and qRT-PCR primers used in gene expression studies.
qRT-PCR for Igκ and Igλ free light chain mRNA
Year: 2016
Region / city: Not specified
Topic: Methods
Document type: Research Methodology
Organization: Not specified
Author: Not specified
Target audience: Researchers in the field of molecular biology
Period of validity: Not specified
Date of approval: Not specified
Date of modification: Not specified
Supplement Table 1: Primers used for qRT-PCR assay
Year: 2026
Type of document: Scientific supplementary material
Field: Molecular biology / Gene expression
Target organisms: Plant genes (specific targets: ACS, ARG7, ERF1, SGR5, RPT3, RPD1, HISTONE H3, ACTIN)
Methods: qRT-PCR assay
Primer type: Gene-specific primers
Amplicon size: 62–193 bp
Unigene IDs: Unigene20575_All, Unigene20551_All, Unigene13079_All, Unigene12164_All, Unigene15061_All, Unigene20360_All, AF304365.1, GQ339772.1
Intended use: Research replication and reference
Supplementary Table S2. Primer sequences for qRT-PCR.
Year: 2026
Note: Region / city
Theme: qRT-PCR primer sequences
Document type: Table
Target audience: Researchers in molecular biology
Context description: A table listing primer sequences for various genes used in qRT-PCR experiments.
Topics: Oithona similis qRT-PCR