№ files_lp_3_process_9_70770
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Experimental methodological record detailing preparation of Lentinus edodes ethanol extract and its biochemical and physiological evaluation in Caenorhabditis elegans through in vitro radical scavenging assays, stress resistance tests, enzyme activity measurements, ROS detection, and apoptosis observation within a controlled laboratory study.
Title: Supplementary Information for Ethanol extract of Lentinus edodes exerted antioxidant activity in Caenorhabditis elegans by regulating insulin DAF-16 signaling pathway
Document Type: Supplementary information
Section: Materials and Methods
Research Topic: Antioxidant activity and insulin DAF-16 signaling pathway
Biological Material: Lentinus edodes
Model Organism: Caenorhabditis elegans (N2)
Experimental Groups: NFD, LEEE-L (0.1 mg/mL), LEEE-M (0.5 mg/mL), LEEE-H (1 mg/mL)
Extraction Method: Ethanol extraction with ultrasonic treatment
In Vitro Assays: DPPH, ABTS, hydroxyl radical scavenging assays
In Vivo Assays: Heat stress assay, acute oxidative stress assay
Biochemical Indicators: MDA, T-SOD, GSH-PX, CAT, ROS
Cell Analysis: Apoptosis assay with acridine orange staining
Reagents and Kits: Beyotime Biotechnology cell lysate; Jiancheng Bioengineering assay kits
Replication: Three independent repeats per experiment
Geographical Reference: Jinggangshan, China; Fuzhou, China; Shanghai, China; Nanjing, China
Storage Conditions: -80°C freezer storage for extract
Price: 8 / 10 USD
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