№ files_lp_4_process_3_071642
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Supplementary scientific material describing experimental analyses of β-arrestin-dependent regulation of EMT-related genes and cellular responses to nicotine in lung cancer cell models.
Document type: Supplementary scientific data
Research field: Molecular biology; cancer biology
Topic: β-arrestin signaling, nicotine response, epithelial–mesenchymal transition (EMT)
Biological context: Non-small cell lung cancer cell lines
Experimental methods: Western blotting, immunofluorescence, qRT-PCR, ChIP assay, transient transfection, wound healing assay, Boyden chamber invasion assay
Analyzed genes and proteins: β-arrestin-1, β-arrestin-2, E-cadherin, ZO1, vimentin, fibronectin, ZEB1, ZEB2, E2F1, Rb
Cell lines: A549, H1650, Calu6, PC9
Experimental factors: Nicotine stimulation, siRNA depletion, shRNA knockdown
Biological processes: Cell migration, invasion, EMT-related gene expression
Promoter analysis: Vimentin and fibronectin promoter regions with E2F binding sites
Associated transcription factors: E2F family members, Rb, p300
Assay targets: Fibronectin and vimentin promoter activity; recruitment of β-arrestin-1 and E2F1
Data presentation: Supplementary figures and experimental descriptions accompanying a primary research study
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