Year:
2023
Region / City:
Cambridge, Massachusetts; Denver, Colorado; Philadelphia, Pennsylvania
Subject:
Pinometostat treatment, leukemia research, resistance mechanisms
Document Type:
Research Methods Supplement
Author(s):
Carly T. Campbell, Jessica N. Haladyna, David A. Drubin, Ty M. Thomson, Michael J. Maria, Taylor Yamauchi, Nigel J. Waters, Edward J. Olhava, Roy M. Pollock, Jesse J. Smith, Robert A. Copeland, Stephen J. Blakemore, Kathrin M. Bernt, Scott R. Daigle
Target Audience:
Researchers, biotechnologists, and medical professionals in oncology and pharmacology
Period of Validity:
2023
Approval Date:
2023
Modification Date:
N/A
Methodology:
Sanger sequencing, RNA-seq, ChIP-Seq, Quantitative PCR, Western Blot, Transporter Study
Context:
Detailed supplemental methods and experimental procedures for studying resistance mechanisms to Pinometostat in MLL rearranged leukemia cells, including sequencing, immunoprecipitation, and transporter studies.
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