№ files_lp_4_process_2_57747
File format: docx Character count: 597 File size: 127 KB
Year: 2026
Region / City: Not specified
Topic: Immunology, oncology
Document Type: Supplementary figure / research data
Institution: Not specified
Author: Not specified
Target Audience: Researchers and clinicians in immunology and oncology
Study Period: Not specified
Date of Data Collection: Not specified
Statistical Methods: Cumulative probit mixed-effects ordinal regressions, SGPV analysis
Biomarkers Measured: HLA-DR, NKG2D, IL-6, IL-8
Sample Type: Peripheral blood and plasma from SD or PD patients
Price: 8 / 10 USD
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Materials and Methods for Supplementary Data: Bioinformatics, RNA Sequencing, Glycosylation Assays, Treg Experiments and Cytokine Detection (analysis completed by December 1, 2023)
Year of completion: 2023
Field: Cancer research and tumor immunology
Research focus: PPP2R2A deletion and expression in NSCLC and immunotherapy response
Type of document: Supplementary materials and methods section of a scientific study
Data sources: cBioPortal Pan-Lung cancer dataset; TCGA pan-cancer RNA-Seq data via Xena Browser; Kmplot survival database
Cell lines: A549 cells; CMT167 PPP2R2A heterozygous knockout cells
Animal models: CD11b-DTR mice (Jackson Laboratory, strain 006000); C57BL6 mice
Experimental techniques: RNA sequencing; Gene Set Enrichment Analysis (GSEA); glycosylation assays with PNGase F and Endo H; adoptive Treg transfer; flow cytometry; Treg suppression assay with CellTrace Violet; ELISA
Repositories: NCBI GEO (accession number GSE311238)
Analytes: PD-L1, TNF-α, IFN-γ, IFN-α, IFN-β
Antibodies and reagents: Anti-Mouse PD-L1 In Vivo Antibody (Clone 10F.9G2, Ichorbio); reagents from BioLegend, STEMCELL Technologies, eBioscience, R&D Systems, PBL Assay Science
Cited works: Gyorffy B., 2024, Innovation (Camb); Qiu Z. et al., 2020, Cancer Research; Zhou L. et al., 2024, Journal of Clinical Investigation
HLA-A, HLA-B and HLA-DR SSP Combi Tray Documentation with Low Resolution Interpretation Tables, Lot 2S1, Expiry 2027-06-01
Year: 2023
Lot Number: 2S1
Expiry Date: 2027-06-01
Document Type: Laboratory test record
Test Type: HLA typing by PCR-SSP
Markers: HLA-A, HLA-B, HLA-DR
Resolution: Low resolution
Sample Data Fields: Name, Sample ID, DNA concentration, Test Date, Tested By, Review Date, Reviewed By, Interpretation, Failed lanes, Comments
Controls: Positive and negative control bands
Data Source: IMGT/HLA database, April 17, 2023, release 3.53.0
Abbreviations Included: Yes
Primer Mix Information: Detailed per HLA locus with amplification notes and potential artifacts
Photo Documentation: Gel images included
Interpretation Guidance: Tables with allele grouping and serological specificity references
Gel Documentation Form and Worksheet for HLA-DQ and HLA-DR Low Resolution Typing
Document type: Laboratory documentation form
Technique: PCR-SSP gel electrophoresis
Genetic system: HLA-DQ and HLA-DR
Resolution level: Low resolution
Product: DQ-DR Combi Tray
Lot number: 6K6
Expiry date: 2024-01-01
Primer sets: HLA-DQ low resolution primer set; HLA-DR low resolution primer set
Control elements: Internal Control Band; negative control primer mix
Allele reference database: IMGT/HLA Database release 3.38.0 (2019-10-17)
Related tables: Interpretation Table; Specificity Table
Measured material: Human genomic DNA
Recorded data: Sample ID; DNA concentration; gel image; interpretation notes
Topics: NKG2D Cytokine HLA-DR