№ files_lp_4_process_2_57437
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Supplementary scientific figure descriptions detailing experimental observations and quantitative analyses of mDia2 and MIRO1 roles in cellular structure, mitochondrial function, and tumor-associated fibroblast activity
Document Type: Supplementary figure legends
Field: Cell biology
Subfield: Mitochondrial dynamics and cytoskeletal regulation
Biological Material: Human primary dermal fibroblasts, CAFs, NFs, keratinocytes, hepatocytes
Proteins Studied: mDia2, MIRO1, GM130, calnexin, α-tubulin, vinculin
Experimental Techniques: Immunofluorescence, Western blot, qRT–PCR, Seahorse analysis, ATP biosensor imaging, PLA assay
Data Type: Quantitative imaging and biochemical analysis
Statistical Methods: One-way ANOVA, two-way ANOVA, Student’s t-test with Bonferroni post-hoc test
Sample Size: n values ranging from 3 to 40 depending on experiment
Key Variables: Mitochondrial network structure, ATP levels, protein expression, fluorescence intensity
Context: Cancer stroma and cellular metabolism
Species: Human
Measurement Units: Micrometers (μm), fluorescence intensity, transcripts per million (TPM)
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Topics: mDia2 MIRO1 S1–S5