№ files_lp_4_process_3_071649
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Character count: 4023
File size: 124 KB
Supplementary scientific methods text describing laboratory procedures for constructing retroviral expression vectors, performing gene knockdown and infection experiments in neural cell models, and analyzing cancer genomic datasets from The Cancer Genome Atlas.
Document type:
Supplementary materials and methods section
Research field:
Molecular biology; cancer genomics
Topic:
Retroviral vector construction, gene knockdown experiments, and genomic data analysis in cancer
Genes studied:
LARP4B; Nf1; p53
Experimental methods:
PCR amplification; retroviral infection; shRNA-mediated knockdown; site-directed mutagenesis
Vectors used:
pMXs-IRES-Puro; pMXs-IRES-Neomycin; MSCV-LTRmiR30-PIG; pSUPER.retro.neo+gfp
Cell types:
U87MG cells; primary astrocytes; immortalized astrocytes; iPS cells
Datasets analyzed:
The Cancer Genome Atlas (TCGA)
Cancer types analyzed:
Glioblastoma; low-grade glioma; acute myeloid leukemia
Data analysis tools:
GISTIC 2.0; Cox regression analysis; two-sample t-test; Benjamini-Hochberg method
Institutions mentioned:
Broad Institute
Referenced publications:
PLoS One (2013); Cold Spring Harbor Protocols (2013); Proceedings of the National Academy of Sciences (2012); Genome Biology (2011); Cancer Discovery (2012)
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The product description is provided for reference. Actual content and formatting may differ slightly.
Source:
TCGA cancer cohort
Section:
Supplementary Materials
Content:
Supplementary figures and table
Methods:
Consensus clustering, pathway enrichment analysis, LASSO regression analysis
Molecular focus:
m6A-related lncRNAs
Number of interactions identified:
625
Number of m6A-related lncRNAs identified:
491
Data description:
Correlation coefficients, p-values, and regulation direction between m6A regulators and lncRNAs
Statistical parameters:
Correlation (cor), p-value, positive and negative regulation
Research field:
Cancer genomics
Type of material:
Supplementary figures and data table
Year:
2026
Database:
TCGA (The Cancer Genome Atlas)
Disease:
Renal cell carcinoma
Document type:
Supplementary table
Sample size:
120+
Parameters:
Overall survival, age, gender, stage, risk score, risk category
Risk model:
Gene combination prognostic model
Stage:
Stage III
Target group:
Patients with advanced RCC
Data format:
Tabular
Section:
Supplemental Methods
Field:
Immunology and Hematologic Malignancies
Experimental focus:
B7-H3 CAR-T cells in acute myeloid leukemia (AML)
Monoclonal antibodies:
Anti-human CD markers and related reagents for flow cytometry
Suppliers:
Becton-Dickinson (BD) Biosciences; Miltenyi Biotec; BioLegend; Jackson ImmunoResearch Labs
Cell lines:
THP1; U937; HL-60; Kasumi-1; OCI-AML2; OCI-AML3
Primary samples:
DFAM-6855; CBAM-44728 from Public Repository of Xenografts (PRoXe), Dana-Farber Cancer Institute
Biological material:
Primary AML blasts; donor PBMCs; buffy coats from healthy volunteers
Vector system:
SFG retroviral vector with MoMLV gag-pol and RD114 envelope
Transduction method:
Retronectin-coated plates with retroviral supernatant
Cell expansion cytokines:
IL7; IL15
Statistical methods:
Mann Whitney test; one-way ANOVA with Holm-Sidak adjustment; Chi-square test
Software:
Prism version 8 (GraphPad)
Sample origin:
Bone marrow and peripheral blood from AML patients
Animal model:
NSG mice for in vivo expansion of xenografts
Culture conditions:
RPMI-1640 medium with fetal bovine serum, L-glutamine, penicillin, and streptomycin
Year:
2020
Region / City:
New South Wales
Subject:
Mathematics
Document Type:
Educational material
Institution:
NSW Education Standards Authority (NESA)
Author:
N/A
Target Audience:
High school students studying Mathematics Extension 1
Period of Use:
N/A
Approval Date:
June 2020
Date of Changes:
N/A
Year:
2019
Region / city:
EU
Theme:
Clinical trials, Genetic modification
Document type:
Application form
Organization / institution:
European Union
Author:
Not specified
Target audience:
Regulatory authorities, developers of medicinal products
Validity period:
Not specified
Approval date:
October 2019
Amendment date:
December 2020
Subject:
Physics
Level:
Secondary education
Topic:
Forces, vectors and scalars
Document type:
Revision worksheet
Content type:
Questions and answers
Key formulas:
W = m × g; W = F × s; F = k × e; a² + b² = c²
Units referenced:
Newton (N); Joule (J); metre (m); kilogram (kg); metre per second (m/s)
Concepts covered:
Scalar quantities; Vector quantities; Resultant force; Work done; Elastic deformation; Gravitational field strength (9.8 N/kg)
Assessment sections:
Basic; Medium; Hard
Year:
2024
Document Type:
Technical Specification
Topic:
Wireless LANs, Secure LTF Key Generation
Organization:
IEEE
Author:
Ali Raissinia
Affiliation:
Qualcomm Inc.
Contact:
[email protected]
Target Audience:
TGme Editor, IEEE 802.11 Working Group
References:
P802.11REVme-D4.2
Transmitter MAC Address:
00:10:18:32:76:54
Year:
Not specified
Level:
Higher IGCSE
Subject:
Mathematics – Vectors
Type:
Revision pack / Exercise questions
Audience:
IGCSE students
Topics covered:
Parallelograms, triangles, trapeziums, column vectors, vector magnitudes, midpoints, ratios, straight lines, hexagons, rectangles
Marks allocation:
Total marks per question indicated
Format:
Questions with diagrams and vector calculations
Period:
Not specified
Year:
2023
Region / city:
Shanghai, China; Poznan, Poland
Subject:
Molecular biology, Genetic engineering
Document type:
Research paper
Institution:
Shanghai Jiao Tong University, Poznan University of Technology
Author:
Liyun Jia, Shuo Xu, Yue Zhang, Muhammad Bilal, Hairong Cheng
Target audience:
Researchers in molecular biology, genetic engineering, and microbiology
Validity period:
Not specified
Approval date:
Not specified
Date of changes:
Not specified
Note:
Year
Note:
Year
Subject:
Mathematics
Document Type:
Educational Assessment
Year:
N/A
Region / city:
N/A
Topic:
Lentiviral vector production
Document type:
Protocol
Organization / institution:
N/A
Author:
N/A
Target audience:
Researchers in molecular biology
Validity period:
N/A
Approval date:
N/A
Date of changes:
N/A
Context description:
Protocol for the production and purification of lentiviral vectors, including detailed steps for DNA transfection, virus collection, and purification using Optiprep.
Unit:
8
Subject:
Mathematics
Topic:
Vectors
Document Type:
Teacher’s Guide
Publication:
Casio ClassWiz Mathematics Workbook
Educational Level:
Secondary Education
Content Includes:
Key Concepts, Laws of Vectors, Column Vectors, Dot Product, Cross Product
Structure:
Explanatory Notes and Worked Examples
Levels Covered:
Level 1, Level 2, Level 3
Tools Referenced:
ClassWiz Calculator
Concepts Covered:
Vector Notation, Vector Addition, Scalar Multiplication, Position Vectors, Magnitude, Angle Between Vectors
Year:
Not specified
Region / City:
Not specified
Subject:
Physics, Mathematics
Document Type:
Educational Worksheet
Organization / Institution:
Not specified
Author:
Not specified
Target Audience:
Students, Physics and Mathematics learners
Period of Validity:
Not specified
Approval Date:
Not specified
Date of Changes:
Not specified
Contextual description:
Educational worksheet on vector addition methods in algebraic, trigonometric, and component form.
Section:
Supplemental Methods
Field:
Immunology and Hematologic Malignancies
Experimental focus:
B7-H3 CAR-T cells in acute myeloid leukemia (AML)
Monoclonal antibodies:
Anti-human CD markers and related reagents for flow cytometry
Suppliers:
Becton-Dickinson (BD) Biosciences; Miltenyi Biotec; BioLegend; Jackson ImmunoResearch Labs
Cell lines:
THP1; U937; HL-60; Kasumi-1; OCI-AML2; OCI-AML3
Primary samples:
DFAM-6855; CBAM-44728 from Public Repository of Xenografts (PRoXe), Dana-Farber Cancer Institute
Biological material:
Primary AML blasts; donor PBMCs; buffy coats from healthy volunteers
Vector system:
SFG retroviral vector with MoMLV gag-pol and RD114 envelope
Transduction method:
Retronectin-coated plates with retroviral supernatant
Cell expansion cytokines:
IL7; IL15
Statistical methods:
Mann Whitney test; one-way ANOVA with Holm-Sidak adjustment; Chi-square test
Software:
Prism version 8 (GraphPad)
Sample origin:
Bone marrow and peripheral blood from AML patients
Animal model:
NSG mice for in vivo expansion of xenografts
Culture conditions:
RPMI-1640 medium with fetal bovine serum, L-glutamine, penicillin, and streptomycin
Year:
2026
Region / City:
Global
Topic:
Programming, Numerical Computing
Document Type:
Tutorial Guide
Organization / Institution:
GNU Octave Project
Author:
Unknown
Target Audience:
Beginners in MATLAB/Octave
Period Covered:
Lesson 1–Lesson 10
Software Version:
Octave / MATLAB compatible
Content Format:
Step-by-step examples with commands and explanations
Includes:
Matrix operations, loops, functions, image and sound handling
Year:
N/A
Region / city:
N/A
Theme:
Gene cloning, CRISPR, Primer design
Document type:
Supplementary file
Organization / institution:
N/A
Author:
N/A
Target audience:
Researchers in molecular biology
Validity period:
N/A
Approval date:
N/A
Modification date:
N/A
Context:
A supplementary document listing sequences of primers for gene cloning and construction of various reporter vectors related to CRISPR-based studies.
Year:
2015
Institution:
Kingsway Christian College
Subject:
Physics
Level:
3AB
Type of document:
Test / Examination Paper
Topics:
Vectors, Projectile Motion, Circular Motion, Gravitational Forces, Satellite Motion
Total Marks:
85
Sections:
Section A – Short Answer, Section B – Calculations
Date:
March 6, 2015
Instructions:
Show all working, use formula book and scientific calculator, answers to 3 significant figures
Audience:
Students of Physics 3AB