№ files_lp_4_process_3_090367
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This document presents a detailed analytical methodology for characterizing the Fc glycosylation of human IgG using immunocapture and LC-MS, aiming to understand the variations in glycosylation profiles related to diseases affecting the immune system.
Year: 2026
Region / city: Brussels, Belgium
Field: Immunology, Glycobiology, Analytical Chemistry
Document type: Research Chapter
Institution: Université libre de Bruxelles
Authors: Yosra Helali, Shilpee Sharma, Marie Vandeput, Dansala Welba, Pierre Van Antwerpen, Arnaud Marchant, Cédric Delporte
Target audience: Researchers, Academics, Healthcare professionals, Immunologists
Period of validity: N/A
Date of approval: N/A
Date of changes: N/A
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Materials and Methods for Supplementary Data: Bioinformatics, RNA Sequencing, Glycosylation Assays, Treg Experiments and Cytokine Detection (analysis completed by December 1, 2023)
Year of completion: 2023
Field: Cancer research and tumor immunology
Research focus: PPP2R2A deletion and expression in NSCLC and immunotherapy response
Type of document: Supplementary materials and methods section of a scientific study
Data sources: cBioPortal Pan-Lung cancer dataset; TCGA pan-cancer RNA-Seq data via Xena Browser; Kmplot survival database
Cell lines: A549 cells; CMT167 PPP2R2A heterozygous knockout cells
Animal models: CD11b-DTR mice (Jackson Laboratory, strain 006000); C57BL6 mice
Experimental techniques: RNA sequencing; Gene Set Enrichment Analysis (GSEA); glycosylation assays with PNGase F and Endo H; adoptive Treg transfer; flow cytometry; Treg suppression assay with CellTrace Violet; ELISA
Repositories: NCBI GEO (accession number GSE311238)
Analytes: PD-L1, TNF-α, IFN-γ, IFN-α, IFN-β
Antibodies and reagents: Anti-Mouse PD-L1 In Vivo Antibody (Clone 10F.9G2, Ichorbio); reagents from BioLegend, STEMCELL Technologies, eBioscience, R&D Systems, PBL Assay Science
Cited works: Gyorffy B., 2024, Innovation (Camb); Qiu Z. et al., 2020, Cancer Research; Zhou L. et al., 2024, Journal of Clinical Investigation
Topics: immunocapture Glycosylation immunoglobulins