№ files_lp_4_process_3_107063
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Supplementary experimental data from a biomedical research study describing antibody characterization, echocardiographic findings in heart failure rat models, and protein expression analyses related to glucocorticoid-mediated regulation of renal sodium and water transport.
Document type: Supplemental research material
Scientific field: Cardiology and renal physiology
Research topic: Effects of glucocorticoids on renal sodium transport in heart failure
Authors: Shuyu Li; Yaomeng Huang; Tongxin Li; Xiaoran Zhu; Weimin Li; Kunshen Liu; Chao Liu
Affiliated institutions: North China University of Science and Technology Affiliated Hospital; The First Hospital of Hebei Medical University; Hebei General Hospital
Country of research: China
Corresponding author: Chao Liu
Experimental subjects: Rat models of chronic heart failure
Methods: Western blot analysis; immunohistochemistry; echocardiography; membrane protein extraction; sodium loading test
Key compounds studied: Dexamethasone (DEX); RU486
Biological targets: ENaC subunits; SGK1; NKCC2; Na⁺/K⁺-ATPase; SGLT1; SGLT2; NaPi-2a; NPRA; V2R; AQP2; AQP3; AQP4; NCC; NHE3
Measured parameters: Fractional excretion of sodium (FeNa%); fractional excretion of potassium (FeK%); protein expression in renal cortex and medulla
Experimental groups: Control rats; chronic heart failure rats; chronic heart failure rats treated with dexamethasone; intervention with RU486
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